A gene replacement strategy for engineering nisin.

doi:10.1099/13500872-142-1-47

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Article

A gene replacement strategy for engineering nisin.

Microbiology (Reading, England) - 1 Jan 1996

Dodd Helen M, Horn Nikki, Giffard Catriona J, Gasson Michael J

Abstract excerpt

A lactococcal expression system was developed which allows the exclusive production of novel nisins encoded by mutated pre-nisin (nisA) genes. This system is based on a combination of a specifically constructed host strain and vectors which facilitate the genetic manipulation of the nisA gene. Th...

Topics

Amino Acid Sequence
Anti-Bacterial Agents
Base Sequence
Chromosomes, Bacterial
Genes, Bacterial
Genetic Variation
Genetic Vectors
Lactococcus
Microbial Sensitivity Tests
Molecular Sequence Data
Mutagenesis, Site-Directed
Nisin
Protein Engineering
Selection, Genetic

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