Article
A cassette vector for protein engineering the lantibiotic nisin.
Gene - 30 Aug 1995
Dodd H M, Horn N, Gasson M J
Abstract excerpt
Expression vectors are described that make use of a plasmid-encoded nisA cassette encoding the peptide component of nisin. Specific mutations can readily be incorporated throughout the coding region of nisA. Using this vector in a nisA mutant host, three variant nisins, with dehydroalanine (Dha) residues changed to Ala residues, were generated.
Topics
- Alanine
- Amino Acid Sequence
- Base Sequence
- Genetic Vectors
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Nisin
- Plasmids
- Protein Engineering