An anticodon sequence mutant of Escherichia coli initiator tRNA: possible importance of a newly acquired base modification next to the anticodon on its activity in initiation.

Abstract excerpt

Initiator tRNAs from eubacteria and chloroplasts lack a base modification next to the anticodon. This is in contrast to virtually all other tRNAs from these sources. We show that a mutant Escherichia coli initiator tRNA which has an anticodon sequence change from CAU to CUA now has a 2-methylthio-N6-(delta 2-isopentenyl)adenosine (ms2i6A) modification, produced by posttranscriptional modification of A, next to...

Topics

• Anticodon
• Bacterial Proteins
• Base Sequence
• DNA-Binding Proteins
• Escherichia coli
• Molecular Sequence Data
• Mutation
• Peptide Chain Initiation, Translational
• Peptide Initiation Factors
• RNA, Transfer, Met