Article
Allele-specific associated polymorphism analysis: novel modification of SSCP for mutation detection in heterozygous alleles using the paradigm of resistance to thyroid hormone.
Human mutation - 1 Jan 1995
Grace M B, Buzard G S, Weintraub B D
Abstract excerpt
Allele-specific polymorphism (ASAP) analysis is a modification of single-strand conformation polymorphism (SSCP) mutation screening under optimized temperature conditions in a minigel format with ethidium bromide detection. ASAP analysis was used to screen for and identify mutations within the hu...
Topics
- Adolescent
- Adult
- Alleles
- Base Sequence
- Child, Preschool
- DNA Mutational Analysis
- Female
- Humans
- Infant
- Male
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Polymorphism, Single-Stranded Conformational
- Thyroid Diseases
- Thyroid Hormones