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Article

Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis.

Applied and environmental microbiology - 1 Nov 1995

Fleming A B, Tangney M, Jørgensen P L, Diderichsen B, Priest F G

Abstract excerpt

A deletion of the spoIIAC gene of Bacillus licheniformis was prepared in vitro by using the splicing-by-overlap-extension technique. This gene was introduced into B. licheniformis on a temperature-sensitive plasmid, and following integration and excision from the chromosome, a precisely located deletion on the chromosomal gene was prepared. The mutated bacterium was totally asporogenous and formed abortively...

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Identifiers

PMID: 8526485
DOI: 10.1128/aem.61.11.3775-3780.1995
PMCID: PMC167678

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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