Article
Characterization of a beta-glucosidase produced by a high-specific growth-rate mutant of Cellulomonas flavigena.
Current microbiology - 1 Apr 2007
Barrera-Islas Gaspar A, Ramos-Valdivia Ana C, Salgado Luis M, Ponce-Noyola Teresa
Abstract excerpt
The mutant strain PN-120 of Cellulomonas flavigena produces a ss-glucosidase that is 10-fold more active than the corresponding enzyme isolated from the parental strain. These enzymes were partially purified through Q Sepharose and Bio-Gel filtration. A single protein band was detected on polyacrylamide-gel electrophoresis/zymogram using 4-methylumbelliferyl-beta-D-glucoside. On sodium dodecyl sulfate-PAGE, the...
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