Effect of Bacillus subtilis spo0A mutation on cell wall lytic enzymes and extracellular proteases, and prevention of cell lysis.

doi:10.1263/jbb.103.13

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Article

Effect of Bacillus subtilis spo0A mutation on cell wall lytic enzymes and extracellular proteases, and prevention of cell lysis.

Journal of bioscience and bioengineering - 1 Jan 2007

Kodama Takeko, Endo Keiji, Ara Katsutoshi, Ozaki Katsuya, Kakeshita Hiroshi, Yamane Kunio, Sekiguchi Junichi

Abstract excerpt

The Bacillus subtilis spo0A mutant is an adequate host for extracellular protein production (e.g., alpha-amylase). However the mutant was prone to cell lysis. SDS-PAGE and zymography of cell wall lytic proteins indicated that the spo0A mutant contained high amounts of two major autolysins (LytC [CwlB] and LytD [CwlG]) and two minor cell wall lytic enzymes (LytE [CwlF] and LytF [CwlE]). On the other hand, the...

Topics

Bacterial Proteins
Bacteriolysis
Cell Membrane
Escherichia coli
Genetic Enhancement
Mutation
N-Acetylmuramoyl-L-alanine Amidase
Peptide Hydrolases
Protein Engineering
Transcription Factors

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PMID: 17298895
DOI: 10.1263/jbb.103.13

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