Article
Cloning and expression of an endocellulase gene from a novel streptomycete isolated from an East African soda lake.
Extremophiles : life under extreme conditions - 1 Oct 2001
van Solingen P, Meijer D, van der Kleij W A, Barnett C, Bolle R, Power S D, Jones B E
Abstract excerpt
Alkaline cellulase-producing actinomycete strains were isolated from mud samples collected from East African soda lakes. The strains were identified as novel Streptomyces spp. by 16S rDNA sequence analysis. A cellulase gene (cel12A) from Streptomyces sp. strain 11AG8 was cloned by expression screening of a genomic DNA library in Escherichia coli. From the nucleotide sequence of a 1.5-kb DNA fragment, an open...
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