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Article

Activation of the phosphosignaling protein CheY. II. Analysis of activated mutants by 19F NMR and protein engineering.

The Journal of biological chemistry - 25 Jun 1993

Bourret R B, Drake S K, Chervitz S A, Simon M I, Falke J J

Abstract excerpt

The Escherichia coli CheY protein is activated by phosphorylation, and in turn alters flagellar rotation. To investigate the molecular mechanism of activation, an extensive collection of mutant CheY proteins was analyzed by behavioral assays, in vitro phosphorylation, and 19F NMR chemical shift measurements. Substitution of a positively charged residue (Arg or Lys) in place of Asp13 in the CheY activation site...

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Identifiers

PMID: 8514750
PMCID: PMC2892986

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Activation of the phosphosignaling protein CheY. II. Analysis of activated mutants by 19F NMR and protein engineering.PMID 8514750Read ↗

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