Article
Role of Position K+4 in the Phosphorylation and Dephosphorylation Reaction Kinetics of the CheY Response Regulator.
Biochemistry - 6 Jul 2021
Foster Clay A, Silversmith Ruth E, Immormino Robert M, Vass Luke R, Kennedy Emily N, Pazy Yael, Collins Edward J, Bourret Robert B
Abstract excerpt
Two-component signaling is a primary method by which microorganisms interact with their environments. A kinase detects stimuli and modulates autophosphorylation activity. The signal propagates by phosphotransfer from the kinase to a response regulator, eliciting a response. Response regulators operate over a range of time scales, corresponding to their related biological processes. Response regulator active site...
Topics
- Allosteric Regulation
- Amino Acid Sequence
- Catalytic Domain
- Escherichia coli
- Escherichia coli Proteins
- Hydrophobic and Hydrophilic Interactions
- Kinetics
- Methyl-Accepting Chemotaxis Proteins
- Molecular Dynamics Simulation
- Mutagenesis, Site-Directed
- Mutation
- Phosphorylation