Article
Conserved aspartate residues and phosphorylation in signal transduction by the chemotaxis protein CheY.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jan 1990
Bourret R B, Hess J F, Simon M I
Abstract excerpt
The CheY protein is phosphorylated by CheA and dephosphorylated by CheZ as part of the chemotactic signal transduction pathway in Escherichia coli. Phosphorylation of CheY has been proposed to occur on an aspartate residue. Each of the eight aspartate residues of CheY was replaced by using site-directed mutagenesis. Substitutions at Asp-12, Asp-13, or Asp-57 resulted in loss of chemotaxis. Most of the mutant CheY...
Topics
- Aspartic Acid
- Bacterial Proteins
- Chemotactic Factors
- Chemotaxis
- Escherichia coli
- Escherichia coli Proteins
- Histidine Kinase
- Kinetics
- Membrane Proteins
- Methyl-Accepting Chemotaxis Proteins