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Hormone- and DNA-binding mechanism of the recombinant human estrogen receptor

22 Jun 1993

Abstract excerpt

We have investigated the hormone- and DNA-binding mechanisms of the wild-type human estrogen receptor (hER) overproduced in insect cells using a baculovirus expression system. The recombinant hER was indistinguishable in size (67 kDa) and immunogenically from the native human estrogen receptor in MCF-7 breast carcinoma cells. The recombinant hER was purified to 70-80% homogeneity with a two-step procedure that...

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PMID: 8512933
DOI: 10.1021/bi00075a016

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