Article
Expression and purification of the extracellular domain of the human follicle-stimulating hormone receptor using Escherichia coli.
The journal of obstetrics and gynaecology research - 1 Feb 2014
Zhang Xiaoyan, Hong Shanshan, Kang Yu, Zheng Yufang, Sun Hong, Xu Congjian
Abstract excerpt
AIM: Although much is known about the structure and biological functions of follicle-stimulating hormone (FSH) receptor (FSHR), the interaction of FSHR and FSH has been challenging to characterize due to the limited quantity of active FSHR protein produced by simple methods. The goal of this study was to express and purify the extracellular domain (ECD) of human FSHR (hFSHR). METHODS: Total RNA was isolated from...
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