Article
The role of estrogen response elements in expression of the Xenopus laevis vitellogenin B1 gene.
Molecular endocrinology (Baltimore, Md.) - 1 Mar 1992
Chang T C, Nardulli A M, Lew D, Shapiro D J
Abstract excerpt
We have used site-directed mutagenesis and a homologous transient transfection system to investigate the role of the two imperfect estrogen response elements (EREs) located at -302/-334 in the 5'-flanking region of the estrogen-regulated Xenopus laevis vitellogenin B1 gene. Deletion of either ERE effectively abolishes estrogen-dependent transcription of the vitellogenin promoter. Neither replacement of the two...
Topics
- Animals
- Base Sequence
- Cell Line
- Cloning, Molecular
- Estrogens
- Female
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed
- Mutation
- Promoter Regions, Genetic