Article
Modified nucleotides in Bacillus subtilis tRNA(Trp) hyperexpressed in Escherichia coli.
Nucleic acids research - 25 May 1993
Xue H, Glasser A L, Desgres J, Grosjean H
Abstract excerpt
In the present study, modified nucleotides in the B. subtilis tRNA(Trp) cloned and hyperexpressed in E. coli have been identified by TLC and HPLC analyses. The modification patterns of the two isoacceptors of cloned B. subtilis tRNA(Trp) have been compared with those of native tRNA(Trp) from B. subtilis and from E. coli. The modifications of the A73 mutant of B. subtilis tRNA(Trp), which is inactive toward its...
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