Article
Species-specific differences in the operational RNA code for aminoacylation of tRNA(Trp).
Nucleic acids research - 15 Oct 2001
Xu F, Chen X, Xin L, Chen L, Jin Y, Wang D
Abstract excerpt
Identity elements play essential roles in the recognition of tRNAs by their cognate aminoacyl-tRNA synthetase. An operational RNA code relates amino acids to specific sequences and structural features of tRNA acceptor stems. In this study, a series of tRNA(Trp) variants was prepared by in vitro transcription and their efficiencies of aminoacylation by tryptophan (k(cat)/K(m)) were measured with the aid of...
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