Article
The td intron endonuclease I-TevI makes extensive sequence-tolerant contacts across the minor groove of its DNA target.
The EMBO journal - 1 May 1993
Bryk M, Quirk S M, Mueller J E, Loizos N, Lawrence C, Belfort M
Abstract excerpt
I-TevI, a double-strand DNA endonuclease encoded by the mobile td intron of phage T4, has specificity for the intronless td allele. Genetic and physical studies indicate that the enzyme makes extensive contacts with its DNA substrate over at least three helical turns and around the circumference of the helix. Remarkably, no single nucleotide within a 48 bp region encompassing this interaction domain is essential...
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