Article
[Expression in Escherichia coli cells of mutant human interferon alpha2].
Molekuliarnaia biologiia - 1 Jan 2000
Ovchinnikov Iu A, Sverdlov E D, Monastyrskaia G S, Tsarev S A, Zaĭtseva E M
Abstract excerpt
cDNA library was obtained from mRNA isolated from human leukocytes induced by Newcastle disease virus. Clones containing cDNA for alpha 2-interferons were identified by colony hybridization with two synthetic hexadecanucleotides. One of the positive clones contained a NH2-terminal part of cDNA of human interferon identical to cDNA for IFN-alpha 2. The only difference between these two clones was the Ser-8 leads...
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