[Expression in Escherichia coli cells of mutant human interferon alpha2].

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Article

[Expression in Escherichia coli cells of mutant human interferon alpha2].

Molekuliarnaia biologiia - 1 Jan 2000

Ovchinnikov Iu A, Sverdlov E D, Monastyrskaia G S, Tsarev S A, Zaĭtseva E M

Abstract excerpt

cDNA library was obtained from mRNA isolated from human leukocytes induced by Newcastle disease virus. Clones containing cDNA for alpha 2-interferons were identified by colony hybridization with two synthetic hexadecanucleotides. One of the positive clones contained a NH2-terminal part of cDNA of...

Topics

Animals
Autoradiography
Base Sequence
Cells, Cultured
Chromosome Mapping
Cloning, Molecular
DNA
DNA, Bacterial
Escherichia coli
Gene Expression Regulation
Genes
Humans
Interferon Type I
Mice
Mutation
Operon
Plasmids
RNA, Messenger

Source

Open in PubMed

Identifiers

PMID: 6369117

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-6369117-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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