Identification of a gene, closely linked to dnaK, which is required for high-temperature growth of Escherichia coli.

Abstract excerpt

We have constructed four deletion derivatives of the cloned dnaK gene. Plasmid pDD1, in which the last 10 amino acids of the DnaK protein have been replaced by three different amino acids derived from the pBR322 vector, was as effective as plasmid pKP31, from which it was derived, in restoring the ability of a dnaK null mutant, Escherichia coli BB1553, to plate lambda phage and to grow at high temperatures. The...

Topics

• Amino Acid Sequence
• Bacterial Proteins
• Bacteriophage lambda
• DNA Transposable Elements
• Escherichia coli
• Escherichia coli Proteins
• Genetic Complementation Test
• HSP70 Heat-Shock Proteins
• Heat-Shock Proteins
• Molecular Sequence Data