Article
Glucose transport activity and photolabelling with 3-[125I]iodo-4-azidophenethylamido-7-O-succinyldeacetyl (IAPS)-forskolin of two mutants at tryptophan-388 and -412 of the glucose transporter GLUT1: dissociation of the binding domains of forskolin and glucose.
The Biochemical journal - 1 Mar 1993
Schürmann A, Keller K, Monden I, Brown F M, Wandel S, Shanahan M F, Joost H G
Abstract excerpt
The tryptophan residues 388 and 412 in the glucose transporter GLUT1 were altered to leucine (L) by site-directed mutagenesis and were transiently expressed in COS-7 cells. As assessed by immunoblotting, comparable numbers of glucose transporters were present in plasma membranes from cells transf...
Topics
- Affinity Labels
- Animals
- Azides
- Binding Sites
- Biological Transport
- Cells, Cultured
- Colforsin
- DNA
- Diterpenes
- Glucose
- Glucose Transporter Type 1
- Monosaccharide Transport Proteins
- Mutation
- Tryptophan