Article
Replacement of both tryptophan residues at 388 and 412 completely abolished cytochalasin B photolabelling of the GLUT1 glucose transporter.
The Biochemical journal - 1 Sept 1994
Inukai K, Asano T, Katagiri H, Anai M, Funaki M, Ishihara H, Tsukuda K, Kikuchi M, Yazaki Y, Oka Y
Abstract excerpt
A mutated GLUT1 glucose transporter, a Trp-388, 412 mutant whose tryptophans 388 and 412 were both replaced by leucines, was constructed by site-directed mutagenesis and expressed in Chinese hamster ovary cells. Glucose transport activity was decreased to approx. 30% in the Trp-388, 412 mutant compared with that in the wild type, a similar decrease in transport activity had been observed previously in the Trp-388...
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