Article
The role of N-glycosylation of GLUT1 for glucose transport activity.
The Journal of biological chemistry - 25 Dec 1991
Asano T, Katagiri H, Takata K, Lin J L, Ishihara H, Inukai K, Tsukuda K, Kikuchi M, Hirano H, Yazaki Y
Abstract excerpt
To elucidate a functional role of N-glycosylation in glucose transporters, we introduced oligonucleotide-directed mutagenesis in GLUT1 cDNA to remove the possible site for N-linked glycosylation. The wild-type and the mutated GLUT1 cDNAs which induced a mutation of Asn at residue 45 to Asp, Tyr, or Gln were transfected and stably expressed into Chinese hamster ovary cells. The expressed wild-type and the mutated...
Topics
- Affinity Labels
- Amino Acid Sequence
- Animals
- Base Sequence
- Biological Transport
- Blotting, Western
- Cells, Cultured
- Cricetinae
- Cricetulus
- Cytochalasin B
- DNA