Article
DNA binding and bending are necessary but not sufficient for Fis-dependent activation of rrnB P1.
Journal of bacteriology - 1 Mar 1993
Gosink K K, Ross W, Leirmo S, Osuna R, Finkel S E, Johnson R C, Gourse R L
Abstract excerpt
The Escherichia coli Fis protein binds to three sites in the upstream activation region of the rrnB P1 promoter and enhances transcription 5- to 10-fold in vivo. In this report, we investigate the mechanism of Fis-dependent activation of transcription. We show that stimulation of rrnB P1 transcri...
Topics
- Carrier Proteins
- DNA, Bacterial
- DNA-Binding Proteins
- Factor For Inversion Stimulation Protein
- Gene Expression Regulation, Bacterial
- Integration Host Factors
- Mutation
- Nucleic Acid Conformation
- Promoter Regions, Genetic
- Recombination, Genetic
- Restriction Mapping
- Transcription, Genetic
- rRNA Operon