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Article

Utility of (His)6 tag for purification and refolding of proplasmepsin-2 and mutants with altered activation properties.

Protein expression and purification - 1 Apr 2002

Gulnik Sergei V, Afonina Elena I, Gustchina Elena, Yu Betty, Silva Abelardo M, Kim Young, Erickson John W

Abstract excerpt

Plasmepsin-2 is a malarial aspartic proteinase that has been implicated in the initial steps of hemoglobin degradation in parasites and thus represents an attractive antimalarial target. Escherichia coli expressed proplasmepsin-2 is capable of activation at acidic pH by autocatalytic cleavage of the pro part region, which results in products of different length. We designed a 10-amino-acid deletion in the pro...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 11922757
DOI: 10.1006/prep.2001.1590

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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