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Article

Functional expression and characterization of a recombinant phospholipase A2 from sea snake Lapemis hardwickii as a soluble protein in E. coli.

Toxicon : official journal of the International Society on Toxinology - 1 May 2003

Yang Wen-Li, Peng Li-Sheng, Zhong Xiao-Fen, Wei Jian-Wen, Jiang Xiao-Yu, Ye Lan-Ting, Zou Lan, Tu Hong-Bin, Wu Wen-Yan, Xu An-long

Abstract excerpt

Three full-length phospholipase A(2) (PLA(2)) cDNAs from sea snake Lapemis hardwickii venom were cloned and sequenced in our previous study. In order to investigate their biological functions, we established a fusion expression system for PLA(2)-9 in E. coli. The open reading frame encoding mature peptide of PLA(2)-9 was subcloned into the vector pTRX. The Trx-PLA(2)-9 fusion protein was expressed as a soluble...

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PMID: 12727275
DOI: 10.1016/s0041-0101(03)00047-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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