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Article

A mutation study of catalytic residue Asp 52 in hen egg lysozyme.

Journal of biochemistry - 1 Jan 1996

Hashimoto Y, Yamada K, Motoshima H, Omura T, Yamada H, Yasukochi T, Miki T, Ueda T, Imoto T

Abstract excerpt

We constructed a system for the expression and secretion of mature hen lysozyme by yeast using an intermediate "secretion-signal cassette" vector, pKP1700, containing the yeast invertase signal sequence and an expression vector, pAM82, for secretion and maturation of the enzyme. Using this system, mutants of hen lysozyme were produced and the catalytic mechanism in hen lysozyme was definitely confirmed. The...

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PMID: 8907188
DOI: 10.1093/oxfordjournals.jbchem.a021199

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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