A mutation study of catalytic residue Asp 52 in hen egg lysozyme.

doi:10.1093/oxfordjournals.jbchem.a021199

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Article

A mutation study of catalytic residue Asp 52 in hen egg lysozyme.

Journal of biochemistry - 1 Jan 1996

Hashimoto Y, Yamada K, Motoshima H, Omura T, Yamada H, Yasukochi T, Miki T, Ueda T, Imoto T

Abstract excerpt

We constructed a system for the expression and secretion of mature hen lysozyme by yeast using an intermediate "secretion-signal cassette" vector, pKP1700, containing the yeast invertase signal sequence and an expression vector, pAM82, for secretion and maturation of the enzyme. Using this system...

Topics

Animals
Aspartic Acid
Base Sequence
Chickens
Crystallography, X-Ray
Egg Proteins
Genetic Vectors
Glycoside Hydrolases
Hydrogen-Ion Concentration
Models, Molecular
Molecular Sequence Data
Muramidase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Plasmids

Protein Conformation

Protein Sorting Signals

Recombinant Proteins

Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 8907188
DOI: 10.1093/oxfordjournals.jbchem.a021199

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8907188-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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