Article
Isolation and characterization of Escherichia coli K-12 mutants lacking both 2-acyl-glycerophosphoethanolamine acyltransferase and acyl-acyl carrier protein synthetase activity.
The Journal of biological chemistry - 25 Jul 1991
Hsu L, Jackowski S, Rock C O
Abstract excerpt
2-Acyl-glycerophosphoethanolamine (2-acyl-GPE) acyltransferase and acyl-acyl carrier protein (acyl-ACP) synthetase are thought to be dual catalytic activities of a single inner membrane enzyme. A filter disc replica print method for the detection of acyl-ACP synthetase activity by colony fluorography was used to screen a mutagenized population of cells for acyl-ACP synthetase mutants (aas). All aas mutants lacked...
Topics
- Acylation
- Acyltransferases
- Chromosome Mapping
- Coenzyme A Ligases
- Escherichia coli
- Fatty Acids
- Genes, Bacterial
- Lysophospholipids
- Membrane Lipids
- Multienzyme Complexes
- Mutation