Article
Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonas reinhardtii.
Molecular & general genetics : MGG - 1 Jan 1993
Dürrenberger F, Rochaix J D
Abstract excerpt
The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-CreI), which is most probably involved in the mobility of this intron. Here we show that I-CreI generates a 4 bp staggered cleavage just downstream of the intron insertion site. The I-Cre...
Topics
- Animals
- Base Sequence
- Chlamydomonas reinhardtii
- Chloroplasts
- DNA Restriction Enzymes
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Genetic Variation
- Introns
- Molecular Sequence Data
- RNA, Ribosomal, 23S
- Recombinant Proteins
- Substrate Specificity