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Article

Isolation and Characterization of ARGONAUTE Mutants in Chlamydomonas.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Yamasaki Tomohito

Abstract excerpt

Random insertional mutagenesis and subsequent reverse genetic screening allow the isolation of mutants of interest. Here I describe the protocol for generating a tag insertion line and subsequent PCR-based screening for ARGONAUTE mutants as an example of a reverse genetic screen for the unicellular green alga Chlamydomonas reinhardtii.

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Identifiers

PMID: 28608341
DOI: 10.1007/978-1-4939-7165-7_11

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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