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Article

In vitro self-splicing reactions of the chloroplast group I intron Cr.LSU from Chlamydomonas reinhardtii and in vivo manipulation via gene-replacement.

Nucleic acids research - 11 Dec 1991

Thompson A J, Herrin D L

Abstract excerpt

The group I intron from the chloroplast rRNA large subunit of Chlamydomonas reinhardtii (Cr.LSU) undergoes autocatalytic splicing in vitro. Cr.LSU displays a range of reactions typical of other group I introns. Under optimal conditions, the 5' cleavage step proceeds rapidly, but the exon-ligation step is relatively slow, and no pH dependent hydrolysis of the 3' splice site occurs. A requirement for high...

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Identifiers

PMID: 1721704
DOI: 10.1093/nar/19.23.6611
PMCID: PMC329230

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