In vitro self-splicing reactions of the chloroplast group I intron Cr.LSU from Chlamydomonas reinhardtii and in vivo manipulation via gene-replacement.

doi:10.1093/nar/19.23.6611

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Article

In vitro self-splicing reactions of the chloroplast group I intron Cr.LSU from Chlamydomonas reinhardtii and in vivo manipulation via gene-replacement.

Nucleic acids research - 11 Dec 1991

Thompson A J, Herrin D L

Abstract excerpt

The group I intron from the chloroplast rRNA large subunit of Chlamydomonas reinhardtii (Cr.LSU) undergoes autocatalytic splicing in vitro. Cr.LSU displays a range of reactions typical of other group I introns. Under optimal conditions, the 5' cleavage step proceeds rapidly, but the exon-ligation step is relatively slow, and no pH dependent hydrolysis of the 3' splice site occurs. A requirement for high...

Topics

Adenosine Triphosphate
Animals
Base Sequence
Blotting, Southern
Chlamydomonas reinhardtii
Chloroplasts
Cloning, Molecular
Endoribonucleases
Hydrogen-Ion Concentration
Introns
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Nucleic Acid Conformation

Nucleotidyltransferases

Open Reading Frames

Plasmids

RNA

Source

Open in PubMed Open full text

Identifiers

PMID: 1721704
DOI: 10.1093/nar/19.23.6611
PMCID: PMC329230

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1721704-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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