Article
Molecular cloning of the yeast OPI3 gene as a high copy number suppressor of the cho2 mutation.
Current genetics - 1 Feb 1993
Preitschopf W, Lückl H, Summers E, Henry S A, Paltauf F, Kohlwein S D
Abstract excerpt
By functional complementation of the auxotrophic requirements for choline of a cdg1, cho2 double-mutant, by transformation with a genomic DNA library in a high copy number plasmid, two different types of complementing DNA inserts were identified. One type of insert was earlier shown to represent the CHO2 structural gene. In this report we describe the molecular and biochemical chemical characterization of the...
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