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Article

Site-directed mutagenesis of proline 204 in the 'hinge' region of yeast phosphoglycerate kinase.

European journal of biochemistry - 1 Feb 1999

McHarg J, Kelly S M, Price N C, Cooper A, Littlechild J A

Abstract excerpt

Site-specific mutants have been produced in order to investigate the role of proline 204 in the 'hinge' region of yeast phosphoglycerate kinase (PGK). This totally conserved proline has been shown to be the only cis-proline in the high resolution crystal structures of yeast, B. stearothermophilus, T. brucei and T. maritima PGK, and may therefore have a role in the independent folding of the two domains or in the...

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Identifiers

PMID: 10092885
DOI: 10.1046/j.1432-1327.1999.00133.x

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