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Article

An alternative mature form of subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis identified in the presence of Ca2+.

The FEBS journal - 1 Jun 2011

Sinsereekul Nitat, Foophow Tita, Yamanouchi Mai, Koga Yuichi, Takano Kazufumi, Kanaya Shigenori

Abstract excerpt

Pro-Tk-SP from Thermococcus kodakaraensis consists of the four domains: N-propeptide, subtilisin (EC 3.4.21.62) domain, β-jelly roll domain and C-propeptide. To analyze the maturation process of this protein, the Pro-Tk-SP derivative with the mutation of the active-site serine residue to Cys (Pro-Tk-S359C), Pro-Tk-S359C derivatives lacking the N-propeptide (ProC-Tk-S359C) and both propeptides (Tk-S359C), and a...

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PMID: 21443525
DOI: 10.1111/j.1742-4658.2011.08107.x

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