Article
Crystallization and preliminary X-ray diffraction study of an active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Sept 2006
Tanaka Shun-ichi, Saito Kenji, Chon Hyongi, Matsumura Hiroyoshi, Koga Yuichi, Takano Kazufumi, Kanaya Shigenori
Abstract excerpt
Crystallization of and preliminary crystallographic studies on an active-site mutant of pro-Tk-subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis were performed. The crystal was grown at 277 K by the sitting-drop vapour-diffusion method. Native X-ray diffraction data were collected to 2.3 A resolution using synchrotron radiation from station BL41XU at SPring-8. The crystal belongs to the...
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