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Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids

1 Mar 1996

Abstract excerpt

We have constructed defined human cytomegalovirus (CMV) mutants by cotransfecting overlapping cosmid clones spanning the 230-kbp genome. Using this strategy, we have introduced a 13-kbp region of DNA from a virulent strain of CMV into a defined position within the avirulent CMV(Towne) genome. Although more than 80% of the genome of these recombinant viruses was derived from Towne DNA, their plaque morphology more...

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PMID: 8627734
DOI: 10.1128/jvi.70.3.2044-2048.1996

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