Article
Rescue of infectivity by in vitro transcapsidation of rotavirus single-shelled particles.
Virology - 1 Feb 1993
Chen D, Ramig R F
Abstract excerpt
We investigated the possibility of rescuing the infectivity of noninfectious single-shelled rotavirus particles by in vitro transcapsidation. The soluble outer capsid proteins VP4 and VP7 were prepared by EDTA treatment of double-shelled (ds) particles of SA11-4F (G serotype 3), followed by removal of single-shelled (ss) particles by three sequential rounds of centrifugation. Ss-particles of B223 (G serotype 10)...
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