Article
Cysteine-102 is positioned in the metal binding activation site of the Corynebacterium diphtheriae regulatory element DtxR.
Proceedings of the National Academy of Sciences of the United States of America - 15 Sept 1993
Tao X, Murphy J R
Abstract excerpt
DNA sequence analysis of dtxR has shown that the M(r) 25,316 regulatory protein contains a single cysteine residue at position 102. DtxR readily forms inactive disulfide-linked dimers. We have used saturation site-directed mutagenesis of the cysteine codon (TGC) at position 102 in order to determ...
Topics
- 2,2'-Dipyridyl
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Corynebacterium diphtheriae
- Cysteine
- DNA, Bacterial
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Genotype
- Iron
- Kinetics
- Molecular Sequence Data
- Molecular Weight