Article
A temperature-sensitive mutation in the acidic polymerase gene of an influenza A virus alters the regulation of viral protein synthesis.
The Journal of general virology - 1 Sept 1993
Herget M, Scholtissek C
Abstract excerpt
The temperature-sensitive defect of mutant ts 263 of fowl plague virus (FPV) is located in the acidic polymerase (PA) gene and is due to a single base substitution (C2036T), which leads to an amino acid replacement (Ala671 to Val) in a highly conserved region of the protein. During passage at 33...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Cells, Cultured
- Chick Embryo
- Cloning, Molecular
- Conserved Sequence
- Crosses, Genetic
- DNA-Directed RNA Polymerases
- Gene Expression Regulation, Viral
- Genes, Viral
- Influenza A virus
- Mutation
- Polymerase Chain Reaction
- RNA, Messenger
- RNA, Viral
- Temperature
- Viral Proteins