Article
Antisense and sense cDNA expression cloning using autonomously replicating vectors and toxic lectin selection.
Biochemical and biophysical research communications - 15 Sept 1993
Cummings L, Warren C E, Granovsky M, Dennis J W
Abstract excerpt
CHOP2 cells, a subline of the Chinese hamster ovary (CHO) cell glycosylation mutant Lec2 which expresses polyoma virus large T antigen, was used as the host cell line to select cDNAs conferring resistance to the toxic effects of ricin. Glycoconjugates in CHOP2 cells are deficient in sialic acid and therefore the cells are hypersensitive to ricin, a galactose-binding lectin. CHOP2 cells acquiring cDNA that either...
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