Article
A simple and efficient method for constructing an adenoviral cDNA expression library.
Molecular therapy : the journal of the American Society of Gene Therapy - 1 Jul 2003
Hatanaka Kazuteru, Ohnami Shumpei, Yoshida Kimiko, Miura Yoshiaki, Aoyagi Kazuhiko, Sasaki Hiroki, Asaka Masahiro, Terada Masaaki, Yoshida Teruhiko, Aoki Kazunori
Abstract excerpt
cDNA expression cloning is a powerful method for the identification of genes that are able to confer a selectable phenotype on specific cell types. An adenovirus vector is characterized by several advantages over plasmid DNA and retroviral vector-mediated gene transfer, such as broad host range and high infectivity. However, an expression cloning protocol using the adenovirus vector has not been reported. We...
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