Article
Molecular cloning of the gene for bilirubin oxidase from Myrothecium verrucaria and its expression in yeast.
1 Sept 1993
Abstract excerpt
Myrothecium verrucaria bilirubin oxidase (EC 1.3.3.5) is an enzyme catalyzing the oxidation of bilirubin to biliverdin and other substrates. We have purified bilirubin oxidase from the medium of M. verrucaria and determined its partial amino acid sequence and isolated cDNA fragment amplified by polymerase chain reaction using oligonucleotide primers designed on the basis of the partial amino acid sequence. The...
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