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Article

Formation of 4-hydroxybenzoate in Escherichia coli: characterization of the ubiC gene and its encoded enzyme chorismate pyruvate-lyase.

Microbiology (Reading, England) - 1 Apr 1994

Siebert M, Severin K, Heide L

Abstract excerpt

Chorismate pyruvate-lyase from Escherichia coli converts chorismate to 4-hydroxybenzoate. The enzyme was enriched 3000-fold by overexpression and chromatographic purification. It has an apparent Km value for chorismate of 6.1 microM and an isoelectric point of pH 6.45. The enzyme activity did not require metal cofactors. Promoter sequences in the 5' flanking sequences of the ubiCA operon were localized by...

Topics

Bacterial Proteins
Base Sequence
Cloning, Molecular
Consensus Sequence
Escherichia coli
Genes, Bacterial
Molecular Sequence Data
Mutation
Oxo-Acid-Lyases
Parabens
Promoter Regions, Genetic

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Identifiers

PMID: 8012607
DOI: 10.1099/00221287-140-4-897

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8012607-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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