Chaperonin-mediated assembly of wild-type and mutant subunits of human propionyl-CoA carboxylase expressed in Escherichia coli.

doi:10.1093/hmg/5.3.331

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Article

Chaperonin-mediated assembly of wild-type and mutant subunits of human propionyl-CoA carboxylase expressed in Escherichia coli.

Human molecular genetics - 1 Mar 1996

Kelson T L, Ohura T, Kraus J P

Abstract excerpt

We developed a bacterial expression system for the human alpha and beta cDNAs of propionyl-CoA carboxylase (PCC). These cDNAs (less the putative mitochondrial matrix targeting presequences) were co-expressed in Escherichia coli on one plasmid vector with each cDNA having its own IPTG-inducible pr...

Topics

Blotting, Western
Carboxy-Lyases
Chaperonin 10
Chaperonins
Escherichia coli
Humans
Methylmalonyl-CoA Decarboxylase
Mutation
Plasmids
Recombinant Proteins

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