Cloning, microbial expression and structure-activity relationship of polyphenol oxidases from Camellia sinensis.

Abstract excerpt

Polyphenol oxidase (PPO) can be used for organic synthesis and degradation of wastes and dyes in industries. Lack of enzyme sources is a major barrier for its application. A PPO gene, with a full length of 1.8kb without introns, was cloned by PCR from genomic DNA of five common cultivars of Camellia sinensis. They had a 98.2-99.9% degree of identity in nucleotides and 94.7-96.1% in amino acids and encoded a...

Topics

• Amino Acid Sequence
• Camellia sinensis
• Catechol Oxidase
• Cloning, Molecular
• Escherichia coli
• Molecular Sequence Data
• Mutation
• Pichia
• Plant Proteins
• Protein Sorting Signals
• Recombinant Proteins