A ribozyme that enhances gene suppression in tobacco protoplasts.

doi:10.1089/ard.1993.3.253

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Article

A ribozyme that enhances gene suppression in tobacco protoplasts.

Antisense research and development - 1 Jan 1993

Perriman R, Graf L, Gerlach W L

Abstract excerpt

Site-directed mutagenesis has been used to produce two hammerhead ribozyme molecules targeting the chloramphenicol acetyltransferase gene (CAT). One ribozyme has a single catalytic domain between two 12-nucleotide arms that can hybridize 5' and 3' of the GUC target site of the CAT RNA transcript....

Topics

Binding Sites
Cells, Cultured
Chloramphenicol O-Acetyltransferase
Electroporation
Mutagenesis, Site-Directed
Mutation
Plants, Toxic
Protoplasts
RNA, Antisense
RNA, Catalytic
RNA, Messenger
Suppression, Genetic
Nicotiana
Transcription, Genetic

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