Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.

Abstract excerpt

Catabolite repression (CR) of xylose utilization by Bacillus subtilis involves a 14-bp cis-acting element (CRE) located in the translated region of the gene encoding xylose isomerase (xylA). Mutations of CRE making it more similar to a previously proposed consensus element lead to increased CR exerted by glucose, fructose, and glycerol. Fusion of CRE to an unrelated, constitutive promoter confers CR to...

Topics

• Aldose-Ketose Isomerases
• Bacillus subtilis
• Base Sequence
• Carbohydrate Epimerases
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Glucose
• Molecular Sequence Data
• Mutation
• Operon