Article
The role of the interdomain B linker in the activation of the XylR protein of Pseudomonas putida.
Molecular microbiology - 1 Oct 2000
Garmendia J, de Lorenzo V
Abstract excerpt
In the presence of toluene and other structural analogues, the enhancer binding protein XylR activates the sigma54 promoter Pu of the TOL (toluene degradation) plasmid pWW0 of Pseudomonas putida. Introduction of amino acid changes Val-219Asp and Ala-220Pro, which enter a proline kink at the interdomain region (B linker) between the A (signal reception) module and the central portion of XylR, originated a protein...
Topics
- Alleles
- Bacterial Proteins
- Culture Media
- DNA-Binding Proteins
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Models, Biological
- Mutagenesis
- Naphthalenes
- Phenotype
- Pseudomonas putida
- Toluene
- Transcription Factors