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Article

An improved method to knock out the asd gene of Salmonella enterica serovar Pullorum.

Journal of biomedicine & biotechnology - 1 Jan 2009

Geng Shi-Zhong, Jiao Xin-An, Pan Zhi-Ming, Chen Xiao-Juan, Zhang Xiao-Ming, Chen Xiang

Abstract excerpt

An asd-deleted (Deltaasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene. Based on the balanced lethal host-vector system, the phenotype of the Deltaasd...

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Identifiers

PMID: 19672470
DOI: 10.1155/2009/646380
PMCID: PMC2723734

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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