Article
Retroviral-mediated gene transfer of a mutant H-ras gene into normal human bone marrow alters myeloid cell proliferation and differentiation.
Experimental hematology - 1 Jan 1994
Maher J, Colonna F, Baker D, Luzzatto L, Roberts I
Abstract excerpt
To investigate the effects of mutant ras expression on the growth and differentiation of normal human bone marrow, we used retrovirus-mediated gene transfer. A retrovirus (HR-1) containing a mutant ras gene (H12-ras) in addition to the selectable neo gene was transferred by cocultivation of a pac...
Topics
- Animals
- Blotting, Northern
- Bone Marrow
- Bone Marrow Cells
- Cell Differentiation
- Cell Division
- Cell Transformation, Viral
- Cells, Cultured
- Gene Transfer Techniques
- Genes, ras
- Hematopoietic Stem Cells
- Humans
- Mice
- Mutation
- Oncogene Protein p21(ras)
- Phenotype
- Retroviridae
- Time Factors