Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Efficient retroviral transduction of human B-lymphoid and myeloid progenitors: marked inhibition of their growth by the Pax5 transgene.

International journal of hematology - 1 May 2008

Sekine Rieko, Kitamura Toshio, Tsuji Takashi, Tojo Arinobu

Abstract excerpt

We applied a coculture system for the genetic manipulation of human B-lymphoid and myeloid progenitor cells using murine bone marrow stromal cell support, and investigated the effects of forced Pax5 expression in both cell types. Cytokine-stimulated cord blood CD34+ cells could be transduced at 85% efficiency and 95% cell viability by a single 24-h infection with RD114-pseudotyped retroviral vectors, produced by...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antigens, CD34
Share to Cell Proliferation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genes, Reporter

Share to Myeloid Progenitor Cells

Share to PAX5 Transcription Factor

Share to Precursor Cells, B-Lymphoid

Share to Retroviridae

Share to Transgenes

Source

Open in PubMed Open full text

Identifiers

PMID: 18415655
DOI: 10.1007/s12185-008-0082-7
PMCID: PMC2668641

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Efficient retroviral transduction of human B-lymphoid and myeloid progenitors: marked inhibition of their growth by the Pax5 transgene.PMID 18415655Read ↗

Constitutive HOXA5 Expression Inhibits Erythropoiesis and Increases Myelopoiesis From Human Hematopoietic ProgenitorsSemantic proximity Read ↗

Biphenotypic B-lymphoid/myeloid cells expressing low levels of Pax5: potential targets of BAL development.Semantic proximity Read ↗

Constitutive HOXA5 Expression Inhibits Erythropoiesis and Increases Myelopoiesis From Human Hematopoietic ProgenitorsSemantic proximity Read ↗

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system.Semantic proximity Read ↗

Human induced pluripotent stem cells are capable of B-cell lymphopoiesisSemantic proximity Read ↗

In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-cultureSemantic proximity Read ↗

Optimization of Fibronectin-Assisted Retroviral Gene Transfer into Human CD34 <sup>+</sup> Hematopoietic CellsSemantic proximity Read ↗

SALL4 is a robust stimulator for the expansion of hematopoietic stem cellsSemantic proximity Read ↗

Highly efficient gene transfer into baboon marrow repopulating cells using GALV-pseudotype oncoretroviral vectors produced by human packaging cellsSemantic proximity Read ↗

Optimization of retroviral gene transfer protocol to maintain the lymphoid potential of progenitor cells.Semantic proximity Read ↗

The BET inhibitor CPI203 promotes ex vivo expansion of cord blood long-term repopulating HSCs and megakaryocytesSemantic proximity Read ↗

Constitutive activation of Flt3 and STAT5A enhances self-renewal and alters differentiation of hematopoietic stem cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.